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Rabbit Anti-TNIP2 antibody (bs-7563R)
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说明书:100ul  200ul
100ul/1380.00元
200ul/2200.00元
大包装/询价

产品编号bs-7563R
英文名称TNIP2
中文名称肿瘤坏死因子α诱导蛋白3相互作用蛋白2抗体
别    名A20 binding inhibitor of NF kappaB activation 2; ABIN 2; FLIP1; KLIP; LKB1 interacting protein; TNFAIP3 interacting protein 2; TNIP2_MOUSE.  
规格价格100ul/1380元购买    200ul/2200元购买    大包装/询价
说 明 书100ul  200ul
研究领域心血管  信号转导  转录调节因子  内皮细胞  表观遗传学  
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Horse, 
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量49kDa
细胞定位细胞核 细胞浆 
性    状Lyophilized or Liquid
浓    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human TNIP2/ABIN2/TNFAIP3 interacting protein 2:85-180/430 
亚    型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The human TNIP2 (also known as ABIN-2), which is related to proteins ABIN-1 and ABIN-3, was originally identified as an A20-associating cytosolic protein that block nuclear factor kappaB (NF-kB) activation. NF-kB plays a central role in the regulation of genes implicated in immune responses, inflammatory processes, and apoptotic cell death and the zinc finger protein A20 is a potent inhibitor of NF-kB activity with a key role in limiting the extent and duration of inflammatory activation. It was also reported that ABIN-2 has the potential to enter the nucleus and plays a role in mediating transcriptional activation in both yeast and mammalian cells.

Function:
Inhibits NF-kappa-B activation by blocking the interaction of RIPK1 with its downstream effector NEMO/IKBKG. Forms a ternary complex with NFKB1 and MAP3K8 but appears to function upstream of MAP3K8 in the TLR4 signaling pathway that regulates MAP3K8 activation. Involved in activation of the MEK/ERK signaling pathway during innate immune response; this function seems to be stimulus- and cell type specific. Required for stability of MAP3K8. Involved in regulation of apoptosis in endothelial cells; promotes TEK agonist-stimulated endothelial survival. May act as transcriptional coactivator when translocated to the nucleus. Enhances CHUK-mediated NF-kappa-B activation involving NF-kappa-B p50-p65 and p50-c-Rel complexes.

Subunit:
nteracts with STK11/LKB1, TNFAIP3, IKBKG, NFKB1, MAP3K8, TEK, RIPK1, CHUK, IKBKB and SMARCD1. Interacts with polyubiquitin

Tissue Specificity:
Ubiquitously expressed in all tissues examined.

Post-translational modifications:
In vitro phosphorylated by CHUK (By similarity).
Ubiquitinated; undergoes 'Lys-48'-linked polyubiquitination probably leading to constitutive proteasomal degradation which can be impaired by IKK-A/CHUK or IKBKB probbaly involving deubiquitination (By similarity).

SWISS:
Q8NFZ5

Gene ID:
79155

Database links:

Entrez Gene: 79155 Human

Entrez Gene: 231130 Mouse

Omim: 610669 Human

SwissProt: Q8NFZ5 Human

SwissProt: Q99JG7 Mouse

Unigene: 726088 Human

Unigene: 28615 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Sample: A431 Cell (Human) Lysate at 40 ug
Primary: Anti-TNIP2 (bs-7563R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TNIP2 Polyclonal Antibody, Unconjugated(bs-7563R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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